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Primary antibodies were incubated for 60 min at room temperature (thioredoxin 1:1000 (#705, American Diagnostica, Stamford, USA), thioredoxin reductase 1:1000 (#07-613, Millipore, Billerica, USA), TxNIP 1:3000 (clone JY2, MBL International Corporation, Woburn, USA), catalase 1:5000 (#ab1877, Abcam, Cambridge, UK), MnSOD 1:1000 (#ab13533, Abcam), GST (clone 2F7) 1:333, GST (clone 2E10-1B2) 1:500 (Abnova, Taipei City, Taiwan), GST 1:2000 (#MSA-102, Assay Designs, Michigan, USA))

Mobile phase A was water with 10 mM ammonium formate and 0.1% formic acid, mobile phase B was 9:1 acetonitrile and water with 10 mM ammonium formate and 0.1% formic acid

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