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Description

GFP was isolated from gWIZ-GFP by PCR that added a separate spacer region and a second hexa-histadine tag (Figure 1A-4)

Nevertheless, standardization of extracts with respect to key active markers such as 4-hydroxyisoleucine and trigonelline remains a necessary step for optimizing clinical efficacy and ensuring reproducibility across future therapeutic applications (149)

J.DsouzaC.ChenG.et al (2010a)

Our single-cell metabolomics approach allowed us to directly probe the metabolism of exhausted CD8 + Tc and to validate a large overdependence on glucose metabolism as a hallmark of CD8 + Tc exhaustion in COVID-19