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Never share an injection pen, even if you changed the needle

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Example 1 Step 1: weighing injection water with the prescription amount of about 30 percent, cooling to 20-30 , and filling high-purity nitrogen into the injection water to replace oxygen in the solution until the oxygen content in the solution is less than 1.5 ppm

Proteins were reduced with 5 mM dithiothreitol (Sigma-Aldrich) for 1 h at RT and alkylated with 10 mM iodoacetamide (Sigma-Aldrich) in the dark for an additional 45 min at RT