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6th edition, January 2020 OptiPrep Application Sheet C21 Dendritic cells from tissues by flotation through a low-density barrier OptiPrep is a 60% (w/v) solution of iodixanol in water, density = 1.32 g/ml The OptiPrep Reference List RC05 Dendritic cells from blood and tissues compares all of the current methodologies and provides a full list of all the published papers reporting the use of OptiPrep: to access return to the initial list of Folders and select Reference Lists To access other Application Sheets referred to in the text return to the Cell Index

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Reaction mix with cell lysate and 1 mM ATP, but no F6P, was monitored as blank control