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Lysates were sonicated and centrifuged for 15 min at 15,000 g

Furthermore, tissue lineage can also influence the metabolic phenotype of a tumour, even when they share the same oncogenic driver mutations (104)

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contacting the first fusion protein with the second fusion protein in binding buffer, wherein a complex between the first fusion protein and the second fusion protein is formed, wherein the binding buffer inhibits a C-terminal protein the invention includes embodiments of fusion proteins comprising a protein of interest (POI) and an intein C-fragment, wherein the POI is fused to the C-terminus of the intein C-fragment, wherein the intein is a naturally split intein DnaE, and the intein C-fragment carries a Asp118Gly mutation within the intein C-fragment

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Cetearyl Alcohol: A fatty alcohol that provides a creamy texture and helps soften the hair