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Flow cytometry was conducted within 1 h with a BD Accuri C6 Plus flow cytometer (San Diego, CA, United States), and the data were analyzed using FlowJo V10 software (BD, San Diego, CA, United States)

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While the number of pericytes is constant in both lines, pericytes of BAC-Syn mice display an activated phenotype, as indicated by immunofluorescence staining for pericyte activation marker NG2 [257, 258]