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In a model of brain injury induced in male rats using a fluid percussion device, researchers noted a significant increase in caspase-3-positive neurons and Neu-N plus TUNEL-positive neurons around the lesion site 72 h post-injury (Tang et al., 2016)

The supernatants were collected and the protein concentration was determined using the Bradford method (Bio-Rad Laboratories, Inc.)

Deng Rong's research group at Sun Yat-sen University first proposed a ferroptosis induction strategy targeting lysosomal exocytosis, providing a new therapeutic direction for Akt-driven refractory tumors [33]