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On February 27, 2026, HHS Secretary Robert F

Elevated levels of TMAO precursors, such as choline and betaine, are also linked to higher CVD prevalence and poor outcomes

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Aliquots at the various time points after fecal slurry inoculation were collected and acidified with 2 mL 0.1N HCl (Sigma-Aldrich, 50%/50% v/v), centrifuged at 4 C at 900 g for 8 min, filter-sterilized in a 0.22-m cellulose acetate filter, and frozen at 80 C until performing the experiments for the determination of the amount of TMA in the fecal slurry supernatants

PMID 30238983

Keep lid tightly sealed., Store in a cool, dry place