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The extraction was done in a buffer [1.0 mm ethylenediaminetetraacetic acid (EDTA), 100 mM TrisHCl (pH 7.3), 100 mM -mercaptoethanol, 1.0 mM phenyl methyl sulfonyl fluoride, 0.1 mM protease inhibitor cocktail, 2% glycerol, 25 mM MgCl 2 , 10 mM dipotassium hydrogen phosphate (K 2 HPO 4 )] and centrifuged at 12,000 g for 15 min, 4 C

Administer RENERVE INJECTION 1ML as follows: The injection will be given by a doctor or nurse in a healthcare setting

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In adults, the global prevalence is estimated at around 6% in individuals under 60 years and up to 20% in those over 60, with rates as high as 7080% reported in low-income regions such as India and parts of Africa due to low intake of animal-based foods