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Reconstitution Volume: Using too little water (e.g., 0.5mL) can make the solution highly concentrated and very difficult to dose accurately in small amounts

RhoA knockdown significantly attenuated IL-1 (100 ng/ml)-induced increase in mRNA expression of ICAM1, VCAM1, MCP-1, TNF-, IL-6 and IFN- (Fig

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The hydrophilic nature and large molecular weight of these molecules prevent passive diffusion across enterocytes, and paracellular transport is restricted by TJ complexes, which typically exclude molecules larger than 1 nm or 600 Da (Shen et al., 2011