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Fully differentiated HepaRG cells (Lonza, catalog: NSHPRG) were plated with 90% of Williams E Medium (Thermo Fisher Scientific, catalog: A1217601) and supplemented with 10% HepaRG thawing and plating medium supplement (Lonza, catalog: MHTAP) and 25 U/mL penicillin-25 g/mL streptomycin

Addressing these gaps would provide a more holistic understanding of organoid functionality across different time points

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No FDA approval: IGF-1 LR3 has never received regulatory approval for human therapeutic use and is restricted to research applications [12]

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