Ensure your thigh muscle is fully relaxed (sit down, do not tense the leg), use room-temperature medication, and insert the needle quickly
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However, individuals who follow vegetarian or vegan diets may be at a higher risk of Vitamin B12 deficiency, as these foods are excluded
2 Materials and methods 2.1 Ethical statement All procedures for experiments were performed following the rules and authorized specifications of the Institutional Animal Care and Use Committee (IACUC), Faculty of Veterinary Medicine, Zagazig University, Egypt under the reference number ZU-IACUC/2/F/195/2022
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