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Reversible inhibition can be further divided into uncompetitive inhibition, which occurs when the inhibitor binds to the enzyme-substrate complex, and mixed-inhibition, when both competitive and uncompetitive occur at the same time ( 3.2 Cannabinoids as regulators of cytochrome P450 metabolic activity Yamaori and colleagues demonstrated through multiple studies using human liver microsomes and recombinant P450: CYP1A1, CYP1A2, CYP1B1 (Yamaori et al., 2010), CYP2A6, CYP2B6 (Yamaori et al., 2011a), CYP2C9 (Yamaori et al., 2012), and CYP2D6 (Yamaori et al., 2011b) that THC and its metabolites are reversible inhibitors of several isoforms of P450