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The day of imaging, 57 m thick sections were made on a cryostat (Leica CM 3050S) on Superfrost Plus Microscope Slides (Fischer Scientific, #12-550-15), incubated for 10 min in Hoechst (1:10 000 in PBS 1) for nuclei visualization, then mounted in a PBS/glycerol 50% (v/v)

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Deoxypodophyllotoxin (DPT), isolated from herbal plants, is used as a precursor for teniposide and etoposide phosphate

Scientists investigate its potential role in cellular signalling, tissue biology, vascular research, and gastrointestinal physiology