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Once inside the cell, ferric iron is released from Tf and free ferric iron is reduced to ferrous iron (Fe2+) by ferric reductase six-transmembrane epithelial antigen of prostate 3 (STEAP3) in the acidic endosome

Protein concentration was determined by BCA assay (ThermoFisher)

After incubation, the solution was removed and 100 l DMSO was added per well

A secondary analysis of the FOURIER trial also demonstrated that, while relative risk for the primary cardiovascular endpoint was consistent across groups, the absolute risk reduction with evolocumab was greatest in patients with elevated hsCRP (458)

doi: 10.1038/s42255-019-0160-6 [DOI] [PMC free article] [PubMed] [Google Scholar] 155.Rosenbaum M, Hall KD, Guo J, Ravussin E, Mayer LS, Reitman ML, et al

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