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Perfusion and tissue preparation Mice were euthanized by IP injection of an overdose of Euthasol (200 g/g), preceded by an injection of a painkiller (Rompun 30 g/g), followed by intracardial perfusion with 25 mL of 2% PBS-paraformaldehyde freshly prepared from 32% PFA solution (Electron Microscopy Sciences, 50-980-495)

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Yes, its safe when administered by trained professionals