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Samples were centrifuged at 20,000 g , 4 C for 30 min, the supernatant transferred to a new tube, centrifuged again at 20,000 g , 4 C for 10 min, and the final supernatant transferred to a new 1.5 ml microtube

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Geng J, Chen X, Wang S, et al

Varicose veins represent a venous disorder that is associated with increases in circulating estrogen (Ciardullo et al., 2000

Importantly, these ROS and RNS can lead to oxidation and nitration of proteins important for the resolution of inflammation

(A) Levels of total GSH (left) or oxidized GSSG (middle) in H