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10.1016/j.bbrc.2016.08.151 Biochem

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While its naturally found in foods like meat and dairy, many people struggle to absorb enough B12 through diet alone, especially those with digestive issues, certain medications, or plant-based lifestyles

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The total reaction mixture consisting of 1.5 ml sodium phosphate buffer (0.1 M, pH 7.5) and PVP (1% w/v) L-methionine (13 mM), enzyme extract (0.1 ml) with same amounts of NBT solution (2.25 mM), riboflavin (60 M), Na 2 CO 3 (1 M), EDTA (3 mM) and double-distilled water (1.0 ml, DDW)