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Diet-induced obese mice on a high-fat diet showed reduced body weight, reduced fat mass, smaller adipocytes, and lower plasma cholesterol with no change in food intake

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MitoSOX red dye (Invitrogen) was used as a probe to monitor mitochondrial superoxide, and Abgreen indicator (Abcam) for H 2 O 2

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Drinking water should contain less that 600mg/L sulfate/L for high concentrate diets, whereas 2,500 mg sulfate/L is acceptable with higher forage intake (NRC 2005.) Generally speaking, sulfur should not exceed 0.3% total diet dry matter

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