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T-bet(+) B cells accumulate in adipose tissue and exacerbate metabolic disorder during obesity

Materials and methods Cell culture Human immortalized keratinocytes (HaCaT) were procured from Bo Gu (Shanghai, China), and cultured in 1640 medium supplemented with 10% FBS and 100 g/mL penicillin and streptomycin at 37 C in air supplemented with 5% CO 2

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