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Although it is hard to ascertain the cause of pyroglutamic acidosis because most of the reported patients had multiple medical comorbidities and/or kidney dysfunction or kidney failure, all of the patients were using APAP

Rodriguez M, Fekry B, Murphy B, Figueroa M, Cheng T, Raber M, Wartenberg L, Bell D, Triche L, Crawford K, Ma H, Allton K, Ahmed R, Tran J, Ranieri C, Konopleva M, Barton M, Nunez C, Eckel-Mahan K, Chandra J

Long-term use has been associated (in some reports) with lower zinc levels

The target proteins for VPA, namely MTOR (PDB ID: 4JT5), PTGS2 (PDB ID: 5KIR), TLR4 (PDB ID: 4G8A) and CBZ namely EGFR (PDB ID: 7KXZ), GSK3B (PDB ID: 5KPL), STAT3 (PDB ID: 6NJS) were retrieved from the RCSB Protein Data Bank

Dulbeccos Modified Eagles Medium (DMEM), fetal bovine serum (FBS), 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA), tert-butyl hydrogen peroxide (t-BHP), N-acetylcysteine (NAC), dimethyl sulfoxide (DMSO), and metaphosphoric acid were all purchased from Sigma-Aldrich (St

10.1271/bbb.64.2402 101 LeeK