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Recombinant methods for the production of stable Fv fragments are known in the art, typically via the insertion of a flexible linker between the light and heavy chain variable domains (to form a single chain Fv (scfv)) or via the introduction of a disulfide bridge between the heavy and light chain variable domains (Strohl, w.r

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( D ) Liver metastatic (PDAC) cell lines stably expressing non-targeting Control, sh Gstt1 or ipCW- Gstt1 in the presence of control shRNA or shRNA targeting FN1 were plated and conditioned media was collected 5 days after dH2O or doxycycline treatment (where indicated)

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