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After a 4-hour incubation, the medium was removed and 1 mL dimethyl sulfoxide was added to each well for cell lysis

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Remember that effective diabetes management requires balancing glycaemic control benefits against quality of life considerations

TNF-alpha - tumour necrosis factor-alpha

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For musculoskeletal applications, this might include pain scores, functional assessments, range of motion measurements, and imaging studies to evaluate structural healing