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Glutathione (GSH) injections are administered subcutaneously (beneath or under all layers of the skin)

For SidC-mediated ubiquitination reaction 49 , 0.3 g GST-E1, 1 g His 6 -UbcH7, 3 g GST-SidC 1-542 and 4 g Ub were incubated in a 20 L reaction system containing 50 mM Tris-HCl (pH 7.5), 5 mM MgCl 2 , 1 mM DTT and 2 mM ATP for 1 h at 37 C

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For the estradiol treatment, 0.3 cm silastic estradiol capsules (Baxter HealthCare, Mundeleine, IL, USA) were implanted subcutaneously in the mice

10.1136/bjophthalmol-2016-309814 133