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All samples were then digested with either 10 g trypsin (4 h at 37C), or with 400 L solution of 1% formic acid in 10% MeOH with 10 g pepsin (1 h at 37C)

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In addition, by feeding quantitative data of bismuth absorption by HK-2 to the SAPT model, we deduce that GSH biosynthesis will be activated after bismuth saturation at 36 h, after which the GSH levels were greatly elevated ( in vivo and in vitro and the timing of the upregulation of GSH levels found in vitro and in silico agree with each other (Figure 4D) ( Upregulation of GSH also accompanies the upregulation of GST activity, and glutathione S-transferase subunit 3A was also found upregulated in bismuth-treated rat kidney cells (Figure 4E) (Figure 5)

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