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281, L31L38

Biphasic response means GHK-Cu shows different effects at different doses

Treatment with MCC950, a selective NLRP3 inhibitor, effectively rescued mitochondrial function and reduced inflammatory activation in both BD and control COs

Accessed 3 Feb

Shaffer et al., 2011)

The resulting protoplasts were then stained using the same procedure as for LP-EVs, involving incubation with DiI dye followed by centrifugation and washing to obtain DiI-labeled 5b4m2