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Of the roughly 2000 CD4 + T cells that can be quantified within the healthy mouse brain, Tregs compose ~150 of them [19]

In the co-culture system, MCECs derived from the model group exhibited significant reduced ZO-1 and Occludin expression compared to those from the Control group, whereas GHK-Cu elevated theses protein levels (Figures 4GI)

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The sections were incubated with primary antibodies at 4 for 24 h, subsequently washed, and then incubated with secondary antibodies at room temperature for 2 h

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