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Since the completion of the Human Genome Project, sequencing methods have undergone dramatic improvements, transitioning from early Sanger sequencing to todays high-throughput next-generation sequencing (NGS) technologies

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Eventually, the protein concentrations were determined by using the BCA assay and diluted to 1 mg/mL with PBS, and 80 L of lysate was used by addition of 20 L of 5 SDS loading buffer and heating at 95 C for 10 min

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intrakeloid), intradermal, and subcutaneous