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PMID: 3 SongXLiangHNanFChenWLiJHeLet al

[21] Meso compounds Meso compounds are stereoisomers that contain two or more chiral centers but are superimposable on their mirror image

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doi: 10.1519/JSC.0b013e318231ac1c

Microinjection was performed using the following injection mix: KCl (25 mmol L 1 ), Hepes pH 7.4 (7.5 mmol L 1 ), tracrRNA (200 ng L 1 ), dpy-10 crRNA (150 ng L 1 ), dpy-10 ssODN (13.75 ng L 1 ), adh-5 sgRNA (300 ng L 1 ), adh-5 ssODN (100 ng L 1 ), Cas9 (416 ng L 1 , NEB, USA) 59