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Finally, the absorbance at 595 nm was recorded by a microplate reader after shaking for 5 min in the dark

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Whereas sulfenic acid and disulfides can be reversed by antioxidant systems such as the thioredoxin (Trx) or glutaredoxin (Grx) systems [231, 232], sulfinic and sulfonic acid are generally considered irreversible modifications in prokaryotes which typically lead to inactivation and aggregation of proteins

The activity of caspase 3, but not caspases 1, 8, or 9, is increased in the BSO-treated E47 cells, and damage to mitochondria appears to play a role in the CYP2E1- and BSO-dependent toxicity, because mitochondrial membrane potential is found to be decreased in the process [124]

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