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Methods This study was approved by the Cummings School of Veterinary Medicine Clinical Studies Review Committee (006.18) and the University of Florida Institutional Animal Care and Use Committee (201,810,504), and all experiments were performed in accordance with their guidelines and regulations

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RNA samples were further processed for mRNA-seq library preparation according to the manufacturers instructions (TruSeq RNA Sample preparation v2, Illumina, San Diego, CA, USA)

125 I/ 131 I-labelled probes for RT exhibited outstanding therapeutic effects

Our results demonstrate that phosphorylation contributes to neuronal toxicity and suggest CK1/ inhibition in combination with other therapies targeting TDP-43 pathology could potentially provide therapeutic benefit in ALS