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Western blotting Cells were lysed in lysis buffer (20 mM Tris, 150 mM NaCl, 1% Nonidet P-40, 1 mM EDTA, 1 mM EGTA, 10% glycerol, and protease and phosphatase inhibitors), incubated on ice for 15 min, and centrifuged at 16,000 x g for 10 min at 4 C

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For example, local clinics like TRUE Addiction and Behavioral Health offer various programs tailored to meet different needs

Human fibroblasts listed in Supplemental Table 2 were cultured in DMEM with 10% FBS plus penicillin and streptomycin

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This suggests that these clinics may contribute to different elements of preoperative optimization that are synergistic