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Laval SH, Timms A, Edwards S, et al

The roots of legume plants house the bacteria, usually in root nodules, in which the microbes convert atmospheric nitrogen into ammonia, thereby allowing the plant to live without added nitrogen fertilizer

GGT catalyses the breakdown of the -glutamyl bond in the substrate and generates p-nitroanilide (pNA), which is monitored kinetically by an increase in absorbance at 418 nm