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Immunofluorescence staining HFF-1 cells (1.5 10 5 cells per well) or mouse embryonic fibroblasts (NIH3T3) were grown on sterile cell slides for 24 h till 80% confluence

Int J Int Med

After parasite pellet resuspension in SDS lysis buffer (50 mm Tris-HCl pH 8, 10 mm EDTA pH 8, 1% SDS), protein quantification was performed with a bicinchoninic acid assay kit (Abcam) and for each condition, 20 g of total proteins were separated on a 12% SDS-PAGE run for 20 min at 100 V, stained with colloidal blue (Thermo Fisher Scientific)

doi: 10.3390/jcm12072482 310 de la PozaGLpez-SanromanATaxoneraCMarn-JimenezIGisbertJPBermejoFet al

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