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Cytotoxicity was assessed by measuring lactate dehydrogenase release as previously described [15] and CSE treated cells under conditions tested did not have changes in cell viability that were statistically significantly different from controls and was greater than 85%

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M1 muscarinic agonists can modulate some of the hallmarks in Alzheimer's disease: implications in future therapy. J Mol Neurosci 20(3): 349-56

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This is largely due to the relatively recent surge in interest in KD for PD