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To further investigate the mechanism underlying STAT3 downregulation, we examined the protein expression levels of its upstream signaling molecule, JAK2, and phosphorylated JAK2 (p-JAK2)

Reactive oxygen species, primarily H 2 O 2, generation, was measured in HCM (7,500 cells per well, 10 M DOX, 4.4 M, B-017/negative control peptide, 4 h), HCF (80,000 cells per well, 500 nM STS, 52 M B-017, 1 h), HEK293 (80,000 cells/ per well, 500 nM STS, 52 M B-017, 1 h) and MCF-7 cells (80,000 cells per well, 500 nM STS, 52 M B-017, 1 h) using the DCFDA/H 2 DCFDACellular ROS Assay Kit (Abcam, ab113851), according to the manufacturers instructions

Tesamorelin primarily targets visceral fat, which is the deep fat stored around internal organs

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