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[Abstract]2022 Aug 5:26:372-386

Tsuda T, Ueno Y, Aoki H, et al

Repeated freeze-thaw cycles, which degrade the peptide structure, must be avoided

2B), GV oocytes recovered via ovum pick up from Holstein heifers were randomly allocated to IVM with or without the same OCM supplementation, which was followed by IVF and IVC in conventional medium up to day 7