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Bioprocessing of recombinant proteins from Escherichia coli inclusion bodies: Insights from structure-function relationship for novel applications

The 3D structures of FPN (ID: 3352) and AST (ID: 5,281,224) were retrieved from the PubChem database ( The 3D structures of protein and ligands were prepared for docking with energy minimization using Chimera 1.16 software 40 while molecular docking was performed using InstaDock software 41 , and the molecular interactions between them were visualized by BIOVIA Discovery Studio Visualizer 2016 software

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But establishing this was not easy at the time because the techniques for doing such experiments were limited

this allows for a time-dependant relationship to be highlighted (Staresinic et al