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doi: 10.1111/ppl.12976 84 KhanM

Bicinchoninic acid assay To quantify the total exosomal protein concentration, an aliquot of stored exosomes was gently thawed on ice and diluted 1:1 (v/v) with ice-cold radioimmunoprecipitation assay (RIPA) lysis buffer, containing protease and phosphatase inhibitors