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Embryos at 48 hpf were stained with 0.6 mg/mL of o -dianisidine (Tokyo Chemical Industry Co., Ltd) for 15 min, followed by fixation with 4% paraformaldehyde (Fujifilm Wako Pure Chemical Corporation)

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Approach 2: The Systemic (Subcutaneous) Strategy The second school of thought leverages one of BPC-157's most fascinating properties: its potential for systemic action