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Eluted peptides were analysed by the MS in positive polarity using a data-independent acquisition mode as follows: an initial MS1 scan was carried out at 140,000 resolution with an automatic gain control target of 3 10 6 for a maximum injection time of 200 ms, m / z range: 4101,600

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The crystal structure of the human NFS1-ISD11-ACP-Zn-ISCU complex shows that the catalytic cysteine of NFS1 is binding to the zinc ion through exchange with Cys35 (Figure 4B