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Sections were fixed in ice-cold 0.5% glutaraldehyde (w/v, PBS) for 15 min and washed with 1 mM MgCl 2 /PBS (pH 6.0) for 5 min

doi: 10.1016/S2213-2600(21)00079-5 176 MacleodMPapiAContoliMBeghBCelliBRWedzichaJAet al

stimulation of endogenous GH production rather than suppressing it

Our findings also suggest that increased non-screen sedentary behaviours and inadequate sleep duration are associated with higher night-time cortisol output