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sativa extract orally every day for 6 weeks to mice caused the death of one mouse following 2 weeks of therapy, while the second and third mice died in the third and fifth weeks after being given 21 and 60 g/kg bw of N

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The mixture was heated at 100 C for 1 h in a water bath after the addition of ninhydrin acid and glacial acetic acid

Abstract Massive production of the free radicals disrupts the system of antioxidant defense in the animal body thereby causing damage to cellular molecules (nucleic acid, lipids, protein, and cell membrane) which results to cell death or mutation that may give rise to abnormal cell division