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The column employed was a Kinetex 1.7 m EVO C18 50 mm x 2.1 mm (Phenomenex, USA) with a quaternary pump equipped with the Agilent 1260 Infinity HPLC Series (Agilent, USA)

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Generation and quantification of protein persulfides and polysulfides in rat heart lysates using Na 2 S 2 Female Wistar rats (obtained from Charles River Laboratories, UK) were housed in pathogen-free facilities with ad libitum access to standard chow and water and were used for experiments at 912 weeks of age

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R.Ocaa-WilhelmiL.Soler-HumanesR.Arranz-SalasI.Garrido-SnchezL.et al