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optimized the structure of darunavir by introducing a 4-(1-hydroxyethyl)phenyl moiety, resulting in the formation of compound 7

This was not possible for the in vitro and in vivo studies, as these experiments were performed by individual investigators who were aware of the experimental groups and treatment outcome

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Cost and Time Constraints Challenge : The GST-tagged protein purification process, while effective, can be resource-intensive in terms of time and cost

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