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The assay can also be adapted to accommodate larger volumes of test sample for readings in a cuvette by using Beers Law and the extinction coefcient of TNB

However, AST-protected cells showed yellow punctum reduction and red fluorescence appearance in the cytoplasm, revealing that AST recovered autophagic flux (Figure 8F)

Article ID 153252, 10 pages

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doi: 10.1371/journal.pone.0254079