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Floro-Styryl-Benzene (FSB) (Dojindo, Tokyo, Japan) staining was carried out according to the manufacturer's instructions

CPZ ( bis -cyclohexanoneoxalyhydrazone) is a well-established demyelinating toxicant, inducing consistent and widespread demyelination in vivo following 6 weeks of daily 0.20.3% CPZ treatment that can be reversed by endogenous remyelination occurring on cessation of treatment (Blakemore, 1972

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Compared to traditional imaging methods, PET offers unique advantages in studying cellular metabolism, including higher spatial resolution and sensitivity