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PRECISE: A prognostic thyrocyte-derived gene signature for papillary thyroid carcinoma

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Biochemistry 50 , 72657274 (2011)

the genes for human IgGl and IgG4 heavy chain constant regions IGHG1 and IGHG44) are amplified using PCR primers containing an Ascl and a Kpnl site ( : GAT CGG TAC CGA TCG GCG CGC CCA AAT CTT GTG ACA AAA CT CAC (SEQ ID NO:54), 4: GAT CGG CGC GCC TTC CAC CAA GGG CCC ATC CGT CTT CCC CCT (SEQ ID NO: 55)) and a Sfil site ( : GAT CGG CCC AGC CGG CCT CAT TTA CCC GGA GAC AGG GAG AGG CTC TTC (SEQ ID NO: 56), 4: GAT CGG CCC AGC CGG CCT CAT TTA CCC AGA GAC AGG GA(SEQ ID NO: 57) primers for amplification of the genes for the human IgE heavy chain constant regions (IGHE) primers can be used containing an Ascl site (GAT CGG CGC GCC CAT CCG TCT TCC CCT TGA (SEQ ID NO:61)), an Sfil site, a 4xhis-tag (GAT CGG CCC AGC CGG CCT CAT TTA CCG GGA TTT ACA GAC AC (SEQ ID NO: 62)), and for the C H 2-4 domains primers containing an Ascl site (GAT CGG CGC GCC CAC CGT GAA GAT CTT AC (SEQ ID NO:63)), an Xbal site, and a 4xhis-tag (GAT CTC TAG ATC AAT GGT GGT GAT GTT TAC CGG GAT TTA CAG ACA CCG (SEQ ID NO: 64)) can be used