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doi: 10.22074/cellj.2019.5992 49

Quantitative PCR analysis Total RNA from 400,000 cells per well was isolated using Trizol reagent according to the manufacturers instructions

The mechanisms of CBDs anticancer effects may involve promoting apoptosis, increasing autophagy, inhibiting the proliferation and migration of tumor cells, counteracting tumor angiogenesis [17], and enhancing the sensitivity of tumor cells to radiotherapy and chemotherapy [18]

Abstract Production stability remains a major challenge in Chinese hamster ovary (CHO) cell-based therapeutic protein manufacturing, particularly during extended passaging where the underlying mechanisms of instability are not fully understood