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In the original protocol, we specified protein expression as primary outcomes, but due to methodological challenges we decided to initially proceed with mRNA analyses

ImageJ 1.53f51 (National Institute of Health, USA) was used to analyze the average red fluorescence intensity within the soma of a motor neuron, while motor neurons themselves were detected in transmitted-light channel

doi: 10.2147/OTT.S254995 13

This dualistic nature underscores the complexity of PNS involvement in pathological states and the need to understand the context-dependent mechanisms that govern its protective versus harmful roles